Welcome to "Explore immunofluorescence"

• This website was made as a small "add on" to my application for a PhD position.
• Within my biochemistry studies, I created a 3D model of cells originating from a z-stack of confocal immunofluorescence images of HUVECs exposed for 24 hours to low laminar fluid shear stress (FSS) of 1 dyne. The model was generated using ImageJ and Blender. It is a reconstruction of a surface around areas of high fluorescence intensity. This means it shows artificially generated surfaces, which are not present within the cell, it is not meant to analyze anything in a scientific way, but just for visualization purposes.
• To play, click on the view below & walk around the cells using W, S, A and D, look around using the trackpad or mouse and jump using space. Can you find some large Caveolin-1 structures (possibly early endosomes) containing Endoglin?

• In blue, a z-stack slice of the nucleus is shown and was generated by DAPI staining of double helical DNA.

• In red, Caveolin-1 was stained. It is a protein which is the main component of caveolae and involved in endocytosis but also integrin signaling.

• In green, Endoglin was stained. Endoglin is a BMP co-receptor important in modulating BMP-signaling, especially in the endothelium during angiogenesis.

• Previously it was observed that differential FSS alters BMP-signaling, therefore we had a look at BMP receptors within this study.
• This project can be adapted to z-stacks of various fluorescence images.